A TWO-HOUR, SINGLE-STEP RT-PCR PROTOCOL FOR DETECTION OF RNA-VIRUS
Autor: | Carl E. Cerniglia, Warren L. Campbell, Rong-Fu Wang |
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Rok vydání: | 1997 |
Předmět: | |
Zdroj: | Journal of Rapid Methods and Automation in Microbiology. 5:321-324 |
ISSN: | 1745-4581 1060-3999 |
DOI: | 10.1111/j.1745-4581.1997.tb00175.x |
Popis: | A rapid protocol of RT-PCR for the detection of RNA-Virus, Mouse Hepatitis Virus, was developed. An Idaho Air-Thermal Cycler was used with 5 mM Mg CI2 in the buffer so that both RT and PCR steps can be run in one tube and the total detection time is less than two hours. |
Databáze: | OpenAIRE |
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