A TWO-HOUR, SINGLE-STEP RT-PCR PROTOCOL FOR DETECTION OF RNA-VIRUS

Autor: Carl E. Cerniglia, Warren L. Campbell, Rong-Fu Wang
Rok vydání: 1997
Předmět:
Zdroj: Journal of Rapid Methods and Automation in Microbiology. 5:321-324
ISSN: 1745-4581
1060-3999
DOI: 10.1111/j.1745-4581.1997.tb00175.x
Popis: A rapid protocol of RT-PCR for the detection of RNA-Virus, Mouse Hepatitis Virus, was developed. An Idaho Air-Thermal Cycler was used with 5 mM Mg CI2 in the buffer so that both RT and PCR steps can be run in one tube and the total detection time is less than two hours.
Databáze: OpenAIRE