Degradation of Rutin into Isoquercitrin by Bacillus litoralis strain C44

Autor:	Shengxun Lin, Zhitang Lu, Yating Zhang, Jinglu Wang
Rok vydání:	2012
Předmět:	chemistry.chemical_compound Transformation (genetics) Hydrolysis Rutin biology Strain (chemistry) Chemistry biology.protein Endoenzyme Food science Microbial biodegradation Quercetin 16S ribosomal RNA
Zdroj:	IOSR Journal of Engineering. :1154-1161
ISSN:	2250-3021 2278-8719
DOI:	10.9790/3021-020511541161
Popis:	To develop the technique of microbial degradation of rutin into isoquercitrin (quercetin 3-glucoside), bacterial strains that could hydrolyze rutin were isolated from Chinese scholar tree Sophora japonica L. soil samples. Strain 44 containing rhanmosidase with the highest transformation ability was obtained after the soil samples were isolated, screened by TLC and re-screened by HPLC. Experiments show that its transformation rate was 50.36% within a 4 mL reaction system containing 50mg wet cells and 0.1% rutin after transforming for 72hr at 25°C. Strain C44 was identified as Bacillus litoralis by integrating the 16S rRNA phylogeny analysis result and a serious of morphological, physiological and biochemical features. The rhanmosidase of strain C44 is a kind of endoenzyme, and the optimal condition of enzyme reaction is 37°C and pH 7.5.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d37c45b04803902cf5a06eb50562d461 https://doi.org/10.9790/3021-020511541161 Zobrazit plný text záznamu