| Popis: |
The results of this investigation on clindamycin and its metabolites support the use of bioautographic thin-layer chromatography (TLC) in metabolite distribution studies. In addition, the use of this technique has established that N-demethylclindamycin contributes significantly to the biological activity of clindamycin in vivo. In spite of the biochemical nature of the results of this study, it should be pointed out that TLC, even in the face of the more sophisticated techniques of gas-liquid chromatography, gas-liquid chromatography-mass spectrometry, and high-pressure liquid chromatography, can still prove to be of considerable value in the solution of certain problems. It is a quick and sensitive method not hampered by the difficulties encountered with the instrumental techniques of separation in terms of interfering substances, particularly when a specific detection system such as bioautography is used. In this light, TLC should still be considered among the more valued analytical techniques. |
