Bioactive Templates Fabricated by Low-Energy Electron Beam Lithography of Self-Assembled Monolayers
| Autor: | K. M. Satyalakshmi, Cindy K. Harnett, Harold G. Craighead |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Materials science Silicon Biomolecule chemistry.chemical_element Nanotechnology Self-assembled monolayer Surfaces and Interfaces Condensed Matter Physics chemistry.chemical_compound Template chemistry Monolayer Electrochemistry General Materials Science Polystyrene Adhesive Spectroscopy Electron-beam lithography |
| Zdroj: | Langmuir. 17:178-182 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la0009543 |
| Popis: | Adhesive templates for biomolecule patterning were fabricated on silicon and gold by low-voltage (1 kV) electron beam lithography of an inert self-assembled monolayer, followed by backfilling the exposed regions with an amine-terminated monolayer. Amine-terminated monolayers selectively attached either the desired materials or linker molecules that subsequently bound other materials including antibodies. Lines (300 nm wide) of 20-nm polystyrene beads were formed on gold by exposing a mercaptohexadecanoic acid (MHDA) monolayer, then backfilling with cysteamine, and selectively attaching aldehyde-coated beads to the amines. Attachment density was found to vary sharply around a critical dose, making the technique useful for patterns such as gradients which require varying density. An optimal dose of 200 μC/cm2 was found for attaching fluorescent polystyrene spheres to MHDA−cysteamine templates. A cycling process was developed for aligning patterns of two or more kinds of polystyrene spheres. Biotin was tethe... |
| Databáze: | OpenAIRE |
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