Association between cycle threshold ( C t ) values and clinical and laboratory data in inpatients with COVID‐19 and asymptomatic health workers
| Autor: | Angel Kaleb Romero-Gonzalez, Yunuen Rodriguez-Sanchez, Victor Javier Cruz-Holguin, Luz Elena Espinosa de los Monteros-Perez, Angélica Olivo-Díaz, Beatriz Zavaleta-Villa, Mirza Romero-Valdovinos, Rigoberto Hernández-Castro, Moisés León-Juárez, Juan Pablo Ramirez-Hinojosa, Lourdes Suarez-Roa, Pablo Maravilla, Guillermina Avila-Ramirez, Octavio Sierra-Martinez, Marisol Chavez-Gutierrez, Aurora Ibarra-Arce, Héctor Manuel Prado-Calleros, Ana Flisser, Sara Arroyo-Escalante, Nelly Raquel Gonzalez-Arenas |
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| Rok vydání: | 2021 |
| Předmět: |
Mechanical ventilation
Cycle threshold Saliva medicine.medical_specialty Coronavirus disease 2019 (COVID-19) business.industry medicine.medical_treatment Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Asymptomatic Virology chemistry.chemical_compound Infectious Diseases chemistry Lactate dehydrogenase Internal medicine Severity of illness medicine medicine.symptom business |
| Zdroj: | Journal of Medical Virology. 93:5969-5976 |
| ISSN: | 1096-9071 0146-6615 |
| Popis: | In-house assays for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), are feasible alternatives, particularly in developing countries. Cycle threshold (Ct ) values obtained by qRT-PCR were compared with clinical and laboratory data from saliva of inpatients with COVID-19 and asymptomatic health workers (AHW) were studied. Saliva specimens from 58 inpatients confirmed by qRT-PCR for SARS-CoV-2 using nasopharyngeal specimens, and 105 AHW were studied by qRT-PCR using three sets of primers for the N (N1, N2, and N3) gene of SARS-CoV-2, according to the CDC Diagnostic Panel protocol, showing a positivity of 88% for inpatients and 8% for AHW. Bivariate analysis revealed an association between Ct < 38.0 values for N2 and mechanical ventilation assistance among patients (p = .013). In addition, values of aspartate-transaminase, lactate dehydrogenase, and ferritin showed significant correlations with Ct values of N1 and N3 genes in inpatients. Therefore, our results show that Ct values correlate with some relevant clinical data for inpatients with COVID-19. |
| Databáze: | OpenAIRE |
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