Preparation of Stable, Granular Formulations ContainingFusarium oxysporumPathogenic to Narcotic Plants

Autor: T.W. Anderson, K.P. Hebbar, R.D. Lumsden, Donald J. Daigle, Armand B. Pepperman, William J. Connick, David Sands
Rok vydání: 1998
Předmět:
Zdroj: Biological Control. 13:79-84
ISSN: 1049-9644
DOI: 10.1006/bcon.1998.0653
Popis: Biomass of formae speciales of Fusarium oxysporum pathogenic to the narcotic plants coca ( Erythroxylum coca ) and opium poppy ( Papaver somniferum ) was incorporated into wheat flour–kaolin granules (Pesta). Shelf life (inoculum viability) of samples with the coca pathogen (strain EN4-S; 2.6 × 10 7 colony-forming units (cfu)/g, mostly chlamydospores) was at least 1 year at 35°C when stored at a water activity ( a W ) of 0.12 (equal to 12% relative humidity) and 2 years when stored at 25°C. Shelf life of samples with the poppy pathogen (strain CP3A; 3.4 × 10 7 cfu/g, mostly microconidia) was about 10 months at 33°C and 0.33 a W . Shelf life was maximized by optimizing water activity (near 0.12 a W for F. oxysporum ) and incorporating at least 5 × 10 6 cfu/g of chlamydospore inoculum. Multikilogram quantities of granular formulations were produced by scaling up the production process by using twin-screw extrusion and fluid-bed drying.
Databáze: OpenAIRE