High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications
| Autor: | Toralf Beitz, Martin Zühlke, Sandro Andreotti, Knut Reinert, Daniel Riebe, Hans-Gerd Löhmannsröben, Marc Diener, Karl Zenichowski |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatography Ion-mobility spectrometry Chemistry Electrospray ionization 010401 analytical chemistry Analytical chemistry Extractive electrospray ionization Filtration and Separation Mass spectrometry 01 natural sciences High-performance liquid chromatography Sample preparation in mass spectrometry 0104 chemical sciences Analytical Chemistry 03 medical and health sciences 030104 developmental biology Liquid chromatography–mass spectrometry Direct electron ionization liquid chromatography–mass spectrometry interface |
| Zdroj: | Journal of Separation Science. 39:4756-4764 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.201600749 |
| Popis: | The combination of high-performance liquid chromatography and electrospray ionization ion mobility spectrometry facilitates the two-dimensional separation of complex mixtures in the retention and drift time plane. The ion mobility spectrometer presented here was optimized for flow rates customarily used in High-performance liquid chromatography between 100–1500 μL min−1. The characterization of the system with respect to such parameters as the peak capacity of each time dimension and of the 2-D spectrum was carried out based on a separation of a pesticide mixture containing 24 substances. While the total ion current chromatogram is coarsely resolved, exhibiting coelutions for a number of compounds, all substances can be separately detected in the 2-D plane due to the orthogonality of the separations in retention and drift dimensions. Another major advantage of the ion mobility detector is the identification of substances based on their characteristic mobilities. Electrospray ionization allows the detection of substances lacking a chromophore. As an example, the separation of a mixture of 18 amino acids is presented. A software built upon the free mass spectrometry package OpenMS was developed for processing the extensive 2-D data. The different processing steps are implemented as separate modules which can be arranged in a graphic workflow facilitating automated processing of data. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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