Aging Results in Molecular Changes in an Enriched Population of Undifferentiated Rat Spermatogonia1
| Autor: | Makoto C. Nagano, Catriona Paul, Bernard Robaire |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
endocrine system
education.field_of_study Testicular atrophy DNA damage Population Cell Biology General Medicine Biology medicine.disease Green fluorescent protein Andrology Transplantation medicine.anatomical_structure Reproductive Medicine Gene expression Immunology medicine education Spermatogenesis Germ cell |
| Zdroj: | Biology of Reproduction. 89 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | A strong correlation exists between increasing paternal age and a decline in reproductive function. Testis aging is associated with testicular atrophy, increased DNA damage, and de novo mutations. It is unclear whether these problems arise from the spermatogonial stem cells (SSCs), a buildup of anomalies as older germ cells progress through spermatogenesis, or both. We hypothesize that with the continual divisions of SSCs that maintain the germ cell population, an alteration of these cells occurs over time. To test this, we utilized young (4-mo-old) and aged (18- and 21-mo-old) transgenic rats that express GFP in germ cells only. We first examined the number and activity of SSCs from the different age groups by transplantation. Aged rats had numerically fewer SSCs than young rats ( |
| Databáze: | OpenAIRE |
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