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Inspite of dramatic improvements in the management of burns, infection still remains a serious risk for the burn patient. Bacterial infection and thermal injury have been shown to trigger the inflammatory response. The aim of this study was to shed light on the impact of acute and subacute burn injury with or without infection on cytokine profiles. Sprague-Dawley rats (n =20) were randomized into three groups: 1) burn only 2) burn and bacteria (106 colony forming units of multi-drug resistant pseudomonas aeruginosa) and 3) sham burn. Animals in the first two groups received a 30% TBSA partial thickness burn. Weight changes were monitored daily. Blood was collected after 12,24,48,72 h and 6 and 12 days, and was used for cytokine ELISAs, LPS quantification, and peripheral blood analysis. Animals were sacrificed either after 6 or 12 days. Skin was harvested for bacterial counts, histological analysis and RNA isolation for microarray analysis. Infected animals showed substantial weight loss until day 6 postburn. We also found higher plasma levels (p |
