In vitro effects of reprogramming factors on the expressions of pluripotent genes and CD34 gene in human acute promyelocytic leukemia HL-60 cells
| Autor: | Xiaohong Yuan, Zhizhe Chen, Jianda Hu, Ling-Yan Wang, Ting Yang, Min Xiao, Yongquan Chen, Xiaofeng Luo, Liang-Fang Zhu, Jinhua Ren |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Acute promyelocytic leukemia fungi HEK 293 cells Transfection Biology medicine.disease Molecular biology Viral vector 03 medical and health sciences 030104 developmental biology SOX2 KLF4 embryonic structures Genetics medicine biological phenomena cell phenomena and immunity Gene Reprogramming |
| Zdroj: | Genomics. 109:331-335 |
| ISSN: | 0888-7543 |
| Popis: | Objective Our study aims to explore the in vitro effects of reprogramming factors on the expressions of pluripotent genes and CD34 gene in HL-60 cells. Methods According to the construction of lentiviral vector LV-OSCK of reprogramming factors (Oct-4, Sox2, Klf4, c-Myc), 293T cells were transfected to detect virus titer. The endogenous pluripotent genes (Oct4, SOX2, c-Myc and Klf4) and CD34 mRNA and protein expressions were detected by AP staining, immunofluorescence staining, qRT-PCR and flow cytometry. Results Expressions of Oct4, SOX2, c-Myc and Klf4 were 0.220 ± 0.013, 0.186 ± 0.009, 0.287 ± 0.015 and 0.153 ± 0.007. These levels were significantly higher in the experimental group than the control and blank groups. CD34 protein expression in the experimental group was also discovered to be significantly higher than the other two groups. Conclusion The reprogramming factors could increase the expressions of pluripotent genes and CD34 gene in HL-60 cells. |
| Databáze: | OpenAIRE |
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