Coordination complexes and catalytic properties of proteins and related substances. 94. Complete amino acid sequence of myoglobin from the pilot whale, Globicephala melaena
| Autor: | Frank R. N. Gurd, Richard A. Bogardt, Barry N. Jones, Margaret Garner, William H. Garner, Lee D. Lehman, Francis E. Dwulet |
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| Rok vydání: | 1978 |
| Předmět: | |
| Zdroj: | Biochemistry. 17:1971-1974 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00603a027 |
| Popis: | The complete amino acid sequence of the major component myoglobin from the pilot whale, Globicephala melaena, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. The apomyoglobin was selectively cleaved at the two methionyl residues with cyanogen bromide and the acetimidated apomyoglobin was cleaved at the three arginyl residues by trypsin. From the sequence analysis of four of these peptides and the apoprotein, over 90% of the covalent structure of the protein was obtained. The remainder of the primary structure was determined by sequence analysis of In preceding papers, the complete amino acid sequence of the myoglobin from Amazon River dolphin (Dwulet et al., 1975), California gray whale (Bogardt et al., 1976), Atlantic bottle- nosed dolphin (Jones et al., 1976), Arctic minke whale (Leh- man et al., 1977), dwarf sperm whale (Dwulet et al., 1977), Pacific common dolphin (Wang et al., 1977) and finback whale (DiMarchi et al., 1978) was reported. All of these sequences of catacean myoglobins were determined by automated Edman degradation. This paper reports the application of the peptide fragmentation and analytical procedures that were used in these papers in determining the complete amino acid sequence of the myoglobin from pilot whale, Globicephala melaena. Completion of this sequence extends the number of complete cetacean myoglobin sequences to 12. In addition to the above-mentioned proteins, the primary structures of the myoglobins from the Black Sea dolphin (Kluh and Bakard- jieva, 1971), common porpoise (Bradshaw and Gurd, 1969), sperm whale (Edmundson, 1965; Romero-Herrera and Lehmann, 1974) and killer whale (Castillo et al., 1977) have also been reported. |
| Databáze: | OpenAIRE |
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