Biomineralization improves the stability of a Streptococcus pneumoniae protein vaccine at high temperatures
| Autor: | Sijie Li, Wenchun Xu, Yajun Min, Jinghui Zhang, Zhaoche Shu, Yibing Yin, Xuemei Zhang, Xiao Yunju, Yusi Liu, Jiangping Meng, Jiangming Xiao |
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| Rok vydání: | 2021 |
| Předmět: |
Biomedical Engineering
Medicine (miscellaneous) chemistry.chemical_element Bioengineering 02 engineering and technology Development Calcium medicine.disease_cause Mineralization (biology) Microbiology 03 medical and health sciences In vivo Streptococcus pneumoniae medicine General Materials Science 030304 developmental biology 0303 health sciences Pneumolysin biology 021001 nanoscience & nanotechnology Proteinase K In vitro chemistry biology.protein 0210 nano-technology Biomineralization |
| Zdroj: | Nanomedicine. 16:1747-1761 |
| ISSN: | 1748-6963 1743-5889 |
| Popis: | Aim: Protein vaccines have been the focus of research for vaccine development due to their safety record and facile production. Improving the stability of proteins is of great significance to the application of protein vaccines. Materials & methods: Based on the proteins pneumolysin and DnaJ of Streptococcus pneumoniae, biomineralization was carried out to prepare protein nanoparticles, and their thermal stability was tested both in vivo and in vitro. Results: Mineralized nanoparticles were formed successfully and these calcium phosphate-encapsulated proteins were resistant to proteinase K degradation and were thermally stable at high temperatures. The mineralized proteins retained the immunoreactivity of the original proteins. Conclusion: Mineralization technology is an effective means to stabilize protein vaccines, presenting a safe and economical method for vaccine administration. |
| Databáze: | OpenAIRE |
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