Evidence of the sterility of allotetraploidCobitisloaches (Teleostei, Cobitidae) using testes ultrastructure
| Autor: | Olga Jablonska, Anna Leska, Radosław Kajetan Kowalski, Alicja Boroń, Dorota Juchno, Anna Pecio, Mirosław Przybylski, Beata Irena Cejko, Sylwia Judycka |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine endocrine system Physiology Zoology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Genetics medicine Molecular Biology Ecology Evolution Behavior and Systematics Cobitidae biology urogenital system fungi food and beverages biology.organism_classification Sertoli cell Spined loach Sperm 030104 developmental biology medicine.anatomical_structure Cobitis Animal Science and Zoology Ploidy Spermatogenesis Germ cell |
| Zdroj: | Journal of Experimental Zoology Part A: Ecological and Integrative Physiology. 327:66-74 |
| ISSN: | 2471-5638 |
| DOI: | 10.1002/jez.2071 |
| Popis: | The diploid-polyploid populations of Cobitis distributed in Poland are usually composed of the spined loach Cobitis taenia or, less often, the Danubian loach C. elongatoides and their triploid (females) and tetraploid hybrids (females and males). The aim of this study was to determine whether tetraploid males participate in the reproduction process by analyzing their testis ultrastructure and the process of spermatogenesis in comparison with diploid males of both parental species. Tetraploid loaches were obtained from three different diploid-polyploid populations distributed in Poland. The structure of Cobitis testes are typical for most Teleostei fish with cystic-type spermatogenesis. The successive stages of developing germ cells are enclosed within cysts formed by the Sertoli cells. This paper morphologically describes the different germ cell stages of spermatogenesis (spermatogonia, spermatocytes, spermatids, and spermatozoa) of C. taenia and C. elongatoides and provides a pioneering ultrastructural analysis of tetraploid Cobitis testes which reveals their unusual structure for the first time. Thus, cysts with normal spermatogonia and spermatocytes (pachyten or leptoten stages) containing synaptonemal complexes were present and no spermatids or spermatozoa were observed. Moreover, in contrast to previously analyzed diploid species, single cells or all of the cells within the cysts displayed chromatin condensation and/or chromatin fragmentation. The obtained results clearly demonstrated that tetraploid males are sterile and diploids are fertile and are the only sperm donors in the reproduction processes of diploid-polyploid Cobitis populations. |
| Databáze: | OpenAIRE |
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