| Autor: |
Tomomasa Watanabe, Tetsuya Mizutani, Masanobu Hayashi, Y. Kon, S. Arai, Futoshi Okada, Akihiko Maeda, Kozue Ishida |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Advances in Experimental Medicine and Biology ISBN: 9781461374329 |
| DOI: |
10.1007/978-1-4615-5331-1_89 |
| Popis: |
We constructed a plasmid expressing mRNA7 coding the nucleocapsid (N) protein of JHM strain of mouse hepatitis virus (JHMV) under the control of Rous sarcoma virus LTR, referred to as pRSV-mRNA7. When C57BL/6 mice injected intramuscularly (i.m.) with control plasmid DNA which contained no viral sequence were infected with JHMV, necrotic figures of neural cells and diffuse immersion of lymphatic cells in the pedunculus cerebri were observed. In the hypothalamus, vascular cuffing consisting of lymphatic cells was observed. In contrast, no histological change was observed throughout these areas of the brains in the JHMV-infected mice after injection with pRSV-mRNA7. These results showed that the injection with plasmid DNA expressing mRNA7 of JHMV partially protected mice against acute infection with JHMV in the brain. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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