A Nitric Oxide Producing Pin-to-Hole Spark Discharge Plasma Enhances Endothelial Cell Proliferation and Migration
| Autor: | Alisa Morss Clyne, Krishna Priya Arjunan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Tube formation
medicine.medical_specialty Materials science Angiogenesis medicine.medical_treatment education Biomedical Engineering General Physics and Astronomy In vitro Cell biology Surgery Nitric oxide Endothelial stem cell chemistry.chemical_compound chemistry hemic and lymphatic diseases medicine Nitrite Wound healing Saline health care economics and organizations |
| Zdroj: | Plasma Medicine. 1:279-293 |
| ISSN: | 1947-5764 |
| DOI: | 10.1615/plasmamed.2012006389 |
| Popis: | Nitric oxide (NO) plays a key role in vascular functions and wound healing. A pin-to-hole spark discharge (PHD), which primarily produces NO, recently emerged as an effective tool for medical applications. We therefore investigated whether PHD plasma-produced NO could promote angiogenesis. PHD plasma equipped with a curved tube extension was used to treat a saline solution and porcine aortic endothelial cells in vitro. Both NO and nitrite increased linearly in plasma-treated phosphate buffered saline, and NO also increased in a dose-dependent manner in endothelial cells. PHD plasma treatment induced endothelial cell proliferation and migration. Cells treated for 60 seconds had 8% more cells than untreated samples 5 days after plasma treatment. A 60-second PHD plasma treatment also increased 2D migration distance by 32% compared to an untreated control, whereas the number of cells that migrated through a 3D collagen gel increased by 16%. However, no tube formation was induced by PHD plasma. These data show that PHD plasma could apply NO to accelerate wound healing through enhanced angiogenesis. |
| Databáze: | OpenAIRE |
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