Fluorescence Analysis of Interferon-Gamma

Autor:	Maya Boyanova, Ivan Ivanov, Roumen Tsanev
Rok vydání:	2007
Předmět:	Chemistry Biological activity Fluorescence Dissociation (chemistry) Dilution Biochemistry Glycation Covalent bond medicine Biophysics Native state Interferon gamma Biotechnology medicine.drug
Zdroj:	Biotechnology & Biotechnological Equipment. 21:215-219
ISSN:	1314-3530 1310-2818
Popis:	Equations were derived describing the fluorescence intensity of IFNy solutions on the basis of a dimer-monomers equilibrium of the native protein. The experimental data were found to fit very well the theoretical values predicted by these equations. Thus they open the possibility of studying the effect of different factors on parameters reflecting the conformational state and the biological activity of IFNy. The dimer-monomers dissociation upon dilution of the interferon solution opens the possibility to assess its native state. The presence and the size of a fraction unable to dissociate and to form active dimers (aggregates due to denaturation or covalent dimers due to glycation) can be revealed by the relative fluorescence decrease upon dilution of the hIFNy solution (“dilution test”).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d2bb99d90f68acb98d33ee566b8b3d2a https://doi.org/10.1080/13102818.2007.10817448 Zobrazit plný text záznamu