p53-mediated negative regulation of stathmin/Op18 expression is associated with G2/M cell-cycle arrest

Autor:	Eric J. Stanbridge, Jean-Francois Cajot, Oscar N. Aurelio, Gunn Eli Kimo Jørgensen, Zeenat Kwaja, Natalia S. Pellegata, John Inge Johnsen, Rina Plattner
Rok vydání:	2000
Předmět:	Regulation of gene expression Cancer Research Differential display Reporter gene Tumor suppressor gene Stathmin macromolecular substances Cell cycle Biology Molecular biology Cell biology Oncology biology.protein Psychological repression Mitosis
Zdroj:	International Journal of Cancer. 88:685-691
ISSN:	1097-0215 0020-7136
Popis:	Utilizing the technique of differential display of mRNA, we have identified p53-responsive genes that are transcriptionally up- or down-regulated as cells enter growth arrest. One gene that was down-regulated, pong16, was found to be identical to stathmin/Op18, a protein involved in the regulation of microtubule dynamics. Evidence that p53 is directly or indirectly involved in negative regulation of stathmin/Op18 expression includes the following: (i) p53-mediated growth inhibition is associated with repression of stathmin/Op18 expression following serum stimulation, (ii) reporter gene assays revealed p53-mediated repression of stathmin/Op18 promoter activity and (iii) constitutive over-expression of stathmin/Op18 bypasses a p53-mediated G2/M arrest in the cell cycle. These results suggest that p53-mediated negative regulation of stathmin/Op18 plays an important role in cell-cycle control. Int. J. Cancer 88:685–691, 2000. © 2000 Wiley-Liss, Inc.
Databáze:	OpenAIRE
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