Phosphotyrosine Antibodies Preferentially React with Basal Epithelial Cells in the Dog Prostate
| Autor: | Alcide Chapdelaine, Simone Chevalier, Louis R. Begin |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
biology Urology Growth factor medicine.medical_treatment Phosphatase medicine.disease Epithelium Cell biology Prostate cancer medicine.anatomical_structure Endocrinology Polyclonal antibodies Internal medicine biology.protein medicine Immunohistochemistry Antibody Signal transduction |
| Zdroj: | Journal of Urology. 155:386-390 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/s0022-5347(01)66665-5 |
| Popis: | Purpose: Considering the hypothesis that androgen-independent but growth factor dependent epithelial cell division may be important in the development and progression of prostate cancer and that protein tyrosine kinases and phosphotyrosine protein phosphatases are key enzymes modulating the levels of specific phosphotyrosylated proteins implicated in several growth factor regulated signal transduction pathways, our aim was to study the cellular distribution of phosphotyrosine proteins in normal and hyperplastic dog prostates as well as in those of castrated dogs supplemented with either androgens or estrogens in order to modify the relative proportion of basal versus secretory epithelial cells.Materials and Methods: Following the determination of optimal conditions to specifically detect phosphotyrosine proteins by a rabbit polyclonal antibody directed against phosphotyrosine, immunohistochemistry was performed on prostate tissue sections from these experimental animals. In addition to morphologic... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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