Choliae esterases and choline acetyltransferase in the seeds ofAllium altaicum (Pall.) Reyse

Autor:	Květa Vacková, J. Hofman, Rita Malini de Almeida, Věra Hadačová, M. Kutáček, Eva Klozová
Rok vydání:	1981
Předmět:	chemistry.chemical_classification Chemistry Plant Science Horticulture Choline acetyltransferase Esterase Butyrylthiocholine chemistry.chemical_compound Enzyme Biochemistry Sinapine Acetylthiocholine medicine Choline Acetylcholine medicine.drug
Zdroj:	Biologia Plantarum. 23:220-227
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02894892
Popis:	In the seeds ofAllium altaicun (Pall.)Reyse a set of enzymes was found, metabolizing choline esters, composed of active choline esterases and choline acetyltransferase. Choline esterase cleaving acetylcholine occurs in five isoenzymes. The enzyme preparation hydrolyses strongly acetylthiocholine and sinapine, but weakly butyrylthiocholine (20%) in comparison with acetylthiocholine. The hydrolysis of the substrates mentioned is inhibited by physostigmine and neostigmine, but it is not inhibited by the specific inhibitor of acetylcholine esterase (BW 284 C51). In addition to hydrolytic activity a strong catalytic activity of choline acetyltransferase was also observed during the synthesis of sinapine from sinapic acid and choline. The detection of the mentioned enzymes in some representatives of theAllium genus indicates that choline esterases are more widely distributed in monocotyledons than previously assumed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d2b53d948f3143b32836c46d996eee20 https://doi.org/10.1007/bf02894892 Zobrazit plný text záznamu Full text from SpringerLink