Optical methods of investigation of the protein Langmuir—Blodgett films
| Autor: | Ilya V. Alexeev, V. V. Savransky, T. B. Dubrovsky, D. E. Akindinov, Alexander P. Savitsky, Oleg I. Lobanov |
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| Rok vydání: | 1995 |
| Předmět: |
biology
Chemistry Metals and Alloys Analytical chemistry Surfaces and Interfaces Laser Langmuir–Blodgett film Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention law Picosecond Materials Chemistry biology.protein Fluorescence microscope Irradiation Bovine serum albumin Image resolution |
| Zdroj: | Thin Solid Films. 259:85-90 |
| ISSN: | 0040-6090 |
| DOI: | 10.1016/0040-6090(94)06431-8 |
| Popis: | The structure of multilayer Langmuir-Blodgett (LB) films made of a protein (bovine serum albumine)-coproporphyrin(III) conjugate was investigated by means of a fluorescence microscope coupled with the time-correlated single-photon counting registration system. The map of coproporphyrin fluorescence from the surface of protein LB films was measured with picosecond time resolution and spatial resolution of 15 μm. The sample area of 225 × 225 μm was irradiated step-by-step by a focused beam of a Nd:YAG laser with the accumulation time of 3 s per point. Two different kinds of samples with a regular and irregular distribution of fluorescent labels over the sample were observed. It is worthwhile to note that the fluorescence level of the studied samples is close to the limit of sensitivity of the fluorescence microscope. Another method to optically investigate the structure of the protein LB films is laser interferometry with consequent computer processing of images. |
| Databáze: | OpenAIRE |
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