Elevated proline content in maize plants expressing a fragment of the proline dehydrogenase gene in antisense orientation

Autor:	M. I. Chumakov, I. V. Volokhina, E. M. Moiseeva, V. A. Velikov, D. A. Agafonov
Rok vydání:	2012
Předmět:	biology fungi food and beverages Plant Science biology.organism_classification Transformation (genetics) Antisense Orientation Exon Proline dehydrogenase Biochemistry Arabidopsis Arabidopsis thaliana Proline Gene
Zdroj:	Russian Journal of Plant Physiology. 59:419-422
ISSN:	1608-3407 1021-4437
DOI:	10.1134/s1021443712020070
Popis:	A fragment of the first exon of the proline dehydrogenase gene of arabidopsis (Arabidopsis thaliana L.) in antisense orientation was transferred to maize (Zea mays L.) genome by agrobacterial transformation in planta to elevate the content of free proline in maize cells. The presence of genetic structure carrying an antisense sequence of the fragment of the proline dehydrogenase gene (ASPG) from the genome of diploid maize seedlings was corroborated by PCR method. T-DNA insertions comprising ASPG were found in the genomes of 30 plants (1.2% of 2409 examined seedlings) belonging to the generation T0. A reliable 4.6-fold increase in proline content was registered in the leaves of transformed maize plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d2a96989a6d490f97e08d98e5fcd18cc https://doi.org/10.1134/s1021443712020070 Zobrazit plný text záznamu Plný text ve formátu PDF