Evaluation of the polyethylene glycol–KF–water system in the context of purifying PEG–protein adducts

Autor:	Yvan Looze, Claudine Paul, Mohamed Azarkan, Anouar Sadat El Moussaoui, Claude Guermant, Jean Vincentelli
Rok vydání:	1999
Předmět:	chemistry.chemical_compound Ammonium sulfate Chromatography chemistry Hydrophilic interaction chromatography PEG ratio Aqueous two-phase system Pharmaceutical Science Salting out Context (language use) Polyethylene glycol Potassium fluoride
Zdroj:	International Journal of Pharmaceutics. 176:241-249
ISSN:	0378-5173
DOI:	10.1016/s0378-5173(98)00323-8
Popis:	Covalent binding of PEG to proteins leads to conjugates widely investigated in several biotechnological processes. Their use as pharmaceuticals requires both careful purification and proper characterization. In this context, this paper examines the potentialities offered by hydrophobic interaction chromatography and compares aqueous potassium fluoride and ammonium sulfate as the eluents. Relative contribution of the various forces which dictate the chromatographic behaviour of PEG–protein adducts on Fractogel TSK–Butyl 650 is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d29fc8e1e9b9590a7e6682598a1f422f https://doi.org/10.1016/s0378-5173(98)00323-8 Zobrazit plný text záznamu Full Text from ScienceDirect