Zum Risiko der Ausbreitung von E. coli/EHEC O104:H4 stx 2 positiven Bakterien über Kläranlagen während der EHEC - Epidemie 2011 in Norddeutschland

Autor:	K. Luden, E.-A. Heinemeyer, M. Monazahian
Rok vydání:	2012
Předmět:	Strain (chemistry) biology 3rd generation cephalosporins business.industry Limit value Public Health Environmental and Occupational Health Outbreak Sewage biology.organism_classification Microbiology Sewerage Sewage treatment business Bacteria
Zdroj:	Das Gesundheitswesen. 75:512-514
ISSN:	1439-4421 0941-3790
DOI:	10.1055/s-0032-1327760
Popis:	During an EHEC outbreak with E. coli O104:H4 stx2-pos in northern Germany 2 sewage treatment plants (Cuxhaven and Stade) of highly affected areas were monitored for the presence of the outbreak strain. 7 efflux water samples were collected at 1 h and 6 h intervals. The overall E. coli content of the treated sewage water was approximately 35 000 CFU/100 mL in both treatment plants. Among these about 500 were ESBL-E. coli (1.4%). ESBL-Agar was used as selective medium as the outbreak strain is highly resistant to 3rd generation cephalosporins. From the ESBL-isolates 208 strains have been typed by molecular methods for markers specific to the outbreak strain (O104rfb -351 base pairs (bp), flic H4-201 bp, stx2-584 bp, Tellur D - 434 bp). No outbreak strain was detected. The number of E. coli O104:H4 stx2-pos was calculated to be less than 3 per 100 mL in the treated sewage at the time of the study. Therefore it can be concluded that there was no threat for bathers to fall sick with this highly pathogenic strain from possibly sewage-contaminated bathing waters during the outbreak. The national limit value of 1 800 E. coli in 100 mL offers a high safety margin.
Databáze:	OpenAIRE
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