High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2

Autor:	B. J. Dzamba, Catherine C. Ridgway, N. L. Charbonneau, R. N. Ono, D. P. Reinhardt, L. Y. Sakai, Douglas R. Keene
Rok vydání:	1998
Předmět:	musculoskeletal diseases congenital hereditary and neonatal diseases and abnormalities Immuno electron microscopy Fibrillin-2 integumentary system Chemistry Biophysics High resolution macromolecular substances skin and connective tissue diseases Instrumentation Fibrillin Fetal skin
Zdroj:	Microscopy and Microanalysis. 4:1162-1163
ISSN:	1435-8115 1431-9276
Popis:	Immunolocalization studies of neonate and older human tissues have previously demonstrated that fibrillin-1 is a component of elastin and non-elastin associated microfibrils. In sections taken from fixed, dehydrated and embedded tissue, microfibrils appear in the transmission electron microscope as hollow rods, 6-8 nm in diameter. When isolated from tissue by homogenization and observed following negative staining or rotary shadowing, microfibrils appear as beaded strings with a degree of extendibility. Recently, a closely related glycoprotein, fibrillin-2, has been described, which is expressed in early fetal development, prior to fibrillin-1, but then disappears in most tissues just prior to birth. We demonstrate here the characterization monoclonal antibodies specific for fibrillin-2. The antibodies are shown by ELISA and immunoblots to be fibrillin-2 specific. The matrix from a variety of tissues, including aorta, tendon, and eye are shown to contain fibrillin-2 by immunofluorescence in a pattern similar to that of fibrillin-1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d29225be992f95703f7eca9ae4f8f0f6 https://doi.org/10.1017/s1431927600025939 Zobrazit plný text záznamu