Plasma 20α-Hydroxypregn-4-en-3-one in the Normal Menstrual Cycle12
| Autor: | Ladislao Prazak, George L. Flickinger, George Mikhail, Chung-Hsiu Wu |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Normal menstrual cycle
medicine.medical_specialty Chemistry Endocrinology Diabetes and Metabolism media_common.quotation_subject Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Luteal phase Biochemistry Endocrinology Column chromatography Sephadex Internal medicine Follicular phase medicine Chromatography column Ovulation media_common |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 39:536-539 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-39-3-536 |
| Popis: | A radioimmunoassay using an antibody against llα,20α-dihydroxypregn-4-en-3-one-11-succinyl-BSA is described for the measurement of 20α-hydroxypregn-4-en-3-one in human plasma. Sephadex LH-20 column chromatography is used to separate progesterone from 20α-OHP. The sensitivity of the assay is 50 pg. The interassay and intra-assay coefficients of variation were 15% and 10%, respectively. Daily measurement of 20α-OHP throughout the normal menstrual cycle revealed levels of 0.41 ± 0.05 ng/ml throughout the follicular phase, followed by a slow rise after ovulation reaching peak levels of 3.4 ± 0.5 ng/ml in the midluteal phase. A constant ratio of 20α-OHP/P of 0.15 to 0.22 was found throughout the luteal phase. Levels in men were not significantly different from those in the early follicular phase of the normal menstrual cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|