Agrobacterium-mediated in planta transformation of maize via pistil filaments

Autor:	M. I. Chumakov, I. V. Volokhina, V. A. Velikov, V. S. Tyrnov, N. A. Rozhok
Rok vydání:	2006
Předmět:	Reporter gene biology Agrobacterium fungi food and beverages Agrobacterium tumefaciens biochemical phenomena metabolism and nutrition medicine.disease_cause biology.organism_classification Genome Transformation (genetics) Seedling Pollen Botany Genetics medicine bacteria Gene
Zdroj:	Russian Journal of Genetics. 42:893-897
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795406080072
Popis:	Integration of T-DNA into the maize genome as a result of treatment of silks with Agrobacterium cells, containing activated vir genes, was demonstrated. In planta treatment of maize (Zea mays L.) was performed during flowering in field. Cell suspension of Agrobacterium tumefaciens line GV3101(pTd33), carrying activated vir genes, was applied onto the previously isolated silks, which were afterwards pollinated with the pollen of the same cultivar. Integration of T-DNA into maize genome was confirmed by PCR (the nptII and gus reporter genes) and hystochemical staining of the seedling tissues, obtained from the transformed seeds. Amplification of the nptII gene showed the presence of about 60.3% of PCR-positive plants out of the total number of kanamycin-resistant seedlings examined, or 6.8% of the total of number of seedlings.
Databáze:	OpenAIRE
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