Indigenous AHL‐degrading bacteriumBacillus firmussw40 affects virulence of pathogenicAeromonas hydrophilaand disease resistance of gibel carp
| Autor: | Kai Chen, Ting Qin, Bingwen Xi, Mingchun Ren, Miao‐Yu Li, Jun Xie |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Biofilm Virulence 04 agricultural and veterinary sciences biochemical phenomena metabolism and nutrition Aquatic Science biology.organism_classification Microbiology 03 medical and health sciences Quorum sensing Aeromonas hydrophila 040102 fisheries Bacillus firmus 0401 agriculture forestry and fisheries Carp Pathogen Bacteria 030304 developmental biology |
| Zdroj: | Aquaculture Research. 50:3755-3762 |
| ISSN: | 1365-2109 1355-557X |
| Popis: | Interrupting quorum sensing represents a novel anti‐infective strategy to combat bacterial pathogen, and biodegradation of quorum sensing signal AHLs has been proved to be an efficient way to control pathogenic Gram‐negative bacteria in aquaculture. In this study, the effect of Bacillus firmus sw40 as efficient AHL‐degrading strain on virulence of fish pathogen Aeromonas hydrophila and disease resistance of gibel carp Carassius auratus gibelio was investigated. The results demonstrated that in vitro the B. firmus sw40 extracellular production (ECP) was able to significantly decrease protease production, haemolytic activity and biofilm formation in A. hydrophila. Dietary administration of B. firmus sw40 (10⁹ CFU/g) for 4 weeks significantly reduced the inflammatory cytokines TNF‐1a, TNF‐2a and IFN‐γ genes expression, antioxidant parameter MDA and GSH levels in serum and increased antioxidant enzyme SOD activity. Besides, B. firmus sw40 could significantly increase the survival of gibel carp with pathogenic A. hydrophila infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|