Steroid receptors in the human prostate
| Autor: | Edward Hawkins, Michelle Nijs, Henri Tagnon, Christiane Brassinne |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Pharmacology
Sucrose medicine.drug_class medicine.medical_treatment Organic Chemistry Clinical Biochemistry Biology Biochemistry Steroid Electrophoresis Cytosol chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Estrogen Prostate medicine Ultracentrifuge Receptor Molecular Biology |
| Zdroj: | Steroids. 26:458-469 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(75)90065-3 |
| Popis: | Agar gel electrophoresis and ultracentrifugation on continuous sucrose gradients revealed the presence of a 4S estradiol ‘receptor’ in cytosols of samples of human benign hyperplastic prostate tissue. High affinity (charcoal stability) and saturability (disappearance with excess competitor) binding characteristics of the molecular species concerned facilitated its clear distinction from similarly migrating serum albumin-steroid complexes. Our data, obtained with human serum, purified human albumin and albumin-enriched cytosol strongly suggest that agar gel electrophoresis, when used alone, may lack specificity for the quantification of estrogen ‘receptors’. Radioligand binding to these molecules may be obscured by similarly migrating albumin-steroid complexes which fall to dissociate during electrophoresis. We advocate the inclusion of competitor experiments to improve the specificity of the method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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