Detection of Lumpy Skin Disease Virus Antigen and Genomic DNA in Formalin-Fixed Paraffin-Embedded Tissues from an Egyptian Outbreak in 2006
Autor: | Shawn Babiuk, Hidefumi Furuoka, Yousef Y. Elseady, Walaa Awadin, H. Hussein |
---|---|
Rok vydání: | 2011 |
Předmět: |
Pathology
medicine.medical_specialty integumentary system General Veterinary General Immunology and Microbiology biology medicine.diagnostic_test Outbreak Histology General Medicine medicine.disease Virology Lumpy skin disease virus law.invention law Lumpy skin disease Biopsy biology.protein medicine Immunohistochemistry Antibody Polymerase chain reaction |
Zdroj: | Transboundary and Emerging Diseases. 58:451-457 |
ISSN: | 1865-1674 |
DOI: | 10.1111/j.1865-1682.2011.01238.x |
Popis: | Summary An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post-mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin-fixed paraffin-embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus-specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions. |
Databáze: | OpenAIRE |
Externí odkaz: |