Detection of Lumpy Skin Disease Virus Antigen and Genomic DNA in Formalin-Fixed Paraffin-Embedded Tissues from an Egyptian Outbreak in 2006

Autor: Shawn Babiuk, Hidefumi Furuoka, Yousef Y. Elseady, Walaa Awadin, H. Hussein
Rok vydání: 2011
Předmět:
Zdroj: Transboundary and Emerging Diseases. 58:451-457
ISSN: 1865-1674
DOI: 10.1111/j.1865-1682.2011.01238.x
Popis: Summary An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post-mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin-fixed paraffin-embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus-specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions.
Databáze: OpenAIRE