A label-free fluorescent probe for accurate mitochondrial G-quadruplex structures tracking via assembly hindered rotation induced emission
| Autor: | Yan-Hong Liu, Jin-Ku Bao, Xiao-Qi Yu, Kun Li, Hui-Zi He, Kang-Kang Yu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Circular dichroism
Chemistry Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics G-quadruplex 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Materials Chemistry Biophysics Nucleic acid Moiety Titration Electrical and Electronic Engineering 0210 nano-technology Selectivity Instrumentation DNA |
| Zdroj: | Sensors and Actuators B: Chemical. 321:128479 |
| ISSN: | 0925-4005 |
| Popis: | Inspired by the mechanism of aggregation induced emission, two derivatives of thiazole orange (TPE-mTO and Ph-TO) were rationally designed and prepared in this work. Their selectivity and sensitivity towards G-quadruplex were studied by fluorescence titration, gel analysis, and circular dichroism (CD) experiments. TPE-mTO could selectively lights-up G-quadruplex DNA structures with no conformational transition, while Ph-mTO could not distinguish the G-quadruplex DNA structure from other nucleic acid, which probably owing to the pocket size and shape of the G-quadruplex DNA only could hinder the rotation of TPE moiety. Then the speculation was verified by molecular docking, TPE-mTO could adopt an appropriate pose in 3′ and 5′ binding pockets of CM22 (G-quadruplex), the multiple interaction between TPE-mTO and CM22 do hinder the rotation of TPE moiety and lead to strong fluorescence. In addition, the detection limit (DL) of TPE-mTO towards CM22 (prefolded G-quadruplex) was found as low as 4.1 nM. With the help of TPE-mTO, the G-quadruplex DNA structures in mitochondrion can be easily and quickly tracked without further washing operations. Overall, the probe we developed (TPE-mTO) was a simple but powerful tool for studying G-quadruplex structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect