Procoagulant Material From Human Urine in Prothrombin Activation

Autor:	Seegers Wh, von Kaulla K, von Kaulla, Caldwell Mj
Rok vydání:	1963
Předmět:	Text mining business.industry Chemistry hemic and lymphatic diseases Hematology Urine Pharmacology business circulatory and respiratory physiology
Zdroj:	Thrombosis and Haemostasis. :053-061
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1654960
Popis:	SummaryThe procoagulant or P fraction obtained from human urine was analyzed to determine how it functions in the activation of purified prothrombin. Prothrombin converts to thrombin in the presence of P fraction, Ac-globulin, calcium chloride and either whole platelets or platelet factor 3. The yield of thrombin can be made to depend upon the concentration of P fraction. This conversion of prothrombin to thrombin can be partially or completely inhibited by adding various concentrations of soybean trypsin inhibitor introduced into the activation mixture.When purified prothrombin was activated with platelet factor 3 and calcium chloride, the prothrombin activity, as measured by the two-stage assay, decreased. When it was at the lowest possible level, P fraction was added. Quite quickly prothrombin activity began to reappear, and soon 100% of the original prothrombin activity was accounted for as either prothrombin-R or thrombin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d237cbb9aa5ad2f5ec1b4f7fba06c3a9 https://doi.org/10.1055/s-0038-1654960 Zobrazit plný text záznamu