Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system
| Autor: | Kelly A. Frazer, C R Brown, J F Burd, K W Higgins, S P Miller, L K Schoelz, V A Marinkovich, J W Dyminski |
|---|---|
| Rok vydání: | 1985 |
| Předmět: | |
| Zdroj: | Clinical Chemistry. 31:1500-1505 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/31.9.1500 |
| Popis: | We have developed a chemiluminescent immunoenzymometric system. The first commercial application of this chemiluminescent assay (CLA) is the measurement of total IgE and allergen-specific IgE in human serum. The CLA system is a second-generation adaptation of the MAST RIA allergy profiling system. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-h incubation with enzyme-labeled antibody, and a 30-min chemiluminescent reaction, which produces a visible image (immunograph) on high-speed Polaroid instant film. The densities of the bands produced on the film are quantified with an inexpensive microprocessor-controlled infrared transmittance densitometer. The novel luminogenic substrates used yield a constant light output for over 2 h with an intensity at least 10-fold greater than that of commercial chemiluminescent reagents. The MAST CLA system exhibits sensitivity, specificity, and precision equal to that of the MAST RIA system (r = 0.96 for 40 serum samples analyzed with 25 allergens). As many as 35 different allergens per sample can be quantified in a single assay. The MAST CLA system requires no standard curve or volume-dependent pipetting steps, incorporates both positive and negative controls for each sample, and quantifies allergen-specific IgE at picomolar concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|