Process optimization for production of a fibrinolytic enzyme from newly isolated marine bacterium Pseudomonas aeruginosa KU1
| Autor: | M. Haridas, A. Sabu, Swaroop S. Kumar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Bioengineering medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound food 010608 biotechnology Skimmed milk medicine Process optimization Food science chemistry.chemical_classification biology Pseudomonas aeruginosa Pseudomonas Factorial experiment biology.organism_classification 030104 developmental biology Enzyme chemistry Tryptone Agronomy and Crop Science Bacteria Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 14:33-39 |
| ISSN: | 1878-8181 |
| Popis: | A potent fibrinolytic enzyme producing bacteria, Pseudomonas aeruginosa KU1 was isolated from marine sediments of Ezhara beach, Kannur, Kerala. Enzyme production was optimized using statistical approach. Placket-Burman factorial design was used in picking key factors (Tryptone, skimmed milk and inoculum size) that influence fibrinolytic enzyme production of the isolate and further optimized using Box-Benhken design. Optimal concentrations for the selected independent variables in the medium were identified to be 0.72% w/v tryptone, 0.09% w/v skimmed milk and 3.95% v/v inoculum size. Peak production was achieved empirically in shake flask culture and it was very close to the projected activity by the response surface model. The Peak production showed 3.25 fold increase over the activity prior to any optimization and a maximum of 1.32 fold increase of one factor at a time optimization. Though many reports are available on fibrinolytic enzyme production from Pseudomonas sp. media optimization studies for enhancement of fibrinolytic enzyme production has not been performed so far. This may be the first report on statistical optimization of production of a fibrinolytic enzyme from marine Pseudomonas sp. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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