Isolation and culture of wapiti (Cervus elaphus) satellite cells
| Autor: | K. M. Byrne, L. Shipley, M. V. Dodson, J. L. Vierck, N. M. Burton |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Canadian Journal of Animal Science. 80:303-309 |
| ISSN: | 1918-1825 0008-3984 |
| DOI: | 10.4141/a99-078 |
| Popis: | Myogenic satellite cells (SC) were isolated from the sternomandibularis muscles of two, 227 kg, male wapiti (Cervus elaphus) and studied in primary cell culture. Wapiti-derived SC were capable of attaching to culture substrata and following the myogenic program of proliferation and differentiation to form multinucleated myotubes. Wapiti SC attached equally well to pig skin gelatin (PSG), fibronectin (FN), Matrigel® and plastic (P > 00.05), but cell viability measured at 120 h varied depending on initial substratum type. Pig skin gelatin (0.02% wt vol−1) was chosen for the majority of subsequent experimentation for cost efficiency. The greatest amount of wapiti SC proliferation was observed in media containing 10% (vol/vol−1) horse serum (HS), 15% HS and 15% fetal bovine serum (FBS) (P > 0.05). Wapiti SC proliferated more when exposed to HS and FBS than to sheep serum (SS) (P 0.05) after 120 h in vitro. Proliferation of SC was doubled when insulin was added to both 10% HS- and 2% HS-containing media (P 0.05). Satellite cells exposed to ITT and ITT-CF differed morphologically from SC exposed to ODM and ODM-Mod, which may suggest that formulation differences are influencing wapiti-derived SC proliferation and differentiation. Key words: Wapiti, satellite cells, primary culture, myotubes |
| Databáze: | OpenAIRE |
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