Zoonotic Potential and Antibiotic Resistance of Escherichia coli in Neonatal Calves in Uruguay
| Autor: | Inés Bado, Ana Umpiérrez, Analía Inés Etcheverría, Nora Lía Padola, Rafael Vignoli, Sofía Acquistapace, Martín Oliver, Pablo Zunino |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology medicine.drug_class 030106 microbiology Antibiotics Soil Science Virulence Plant Science General Medicine Integron medicine.disease_cause Microbiology 03 medical and health sciences 030104 developmental biology Antibiotic resistance biology.protein medicine Multilocus sequence typing MCR-1 Escherichia coli Ecology Evolution Behavior and Systematics Intimin |
| Zdroj: | Microbes and Environments. 32:275-282 |
| ISSN: | 1347-4405 1342-6311 |
| Popis: | Escherichia coli is one of the main etiological agents of neonatal calf diarrhea (NCD). The objective of this study was to assess the presence of virulence genes, genetic diversity, and antibiotic resistance mechanisms in E. coli associated with NCD in Uruguay. PCR was used to assess the presence of intimin, Shiga-like toxin, and stable and labile enterotoxin genes. Resistance to fluoroquinolones and oxyimino-cephalosporins was estimated on Muller-Hinton agar plates. Further antibiotic disc-diffusion tests were performed to assess bacterial multi-resistance. The presence of PMQR, ESBL, MCR-1, and integron genes was evaluated. Isolates were typed using ERIC-PCR, and 20 were selected for MLST, adhesion to Hep-2 cells, in vitro biofilm formation, and eukaryotic cytotoxicity. The prevalence of ETEC genes was lower than 3% in each case (estA and elt). Six isolates were EPEC (eae+) and 2 were EHEC/STEC (eae+/stx1+). The results of a diversity analysis showed high genetic heterogenicity among isolates. Additionally, different sequence types, including ST10, ST21, and ST69, were assigned to selected isolates. Thirty-six percent (96/264) of the isolates were fluoroquinolone-resistant, with 61/96 (63.5%) being multidrug-resistant. Additionally, 6 were oxyimino-cephalosporin-resistant. The qnrB, qnrS1, and blaCTX-M-14 genes were detected, whereas no isolates carried the mcr-1 gene. Isolates had the ability to adhere to Hep-2 cells and form biofilms. Only 1 isolate expressed toxins in vitro. E. coli from NCD cases in Uruguay are very diverse, potentially virulent, and may interact with eukaryotic cells. Zoonotic potential, together with resistance traits and the presence of horizontal transfer mechanisms, may play a significant role in infections caused by these microorganisms. |
| Databáze: | OpenAIRE |
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