Acrylamide Optical Sensor Based on Hydrolysis Using Bacillus sp. Strain ZK34 Containing Amidase Properties
| Autor: | Norzila Kusnin, Yee May Chong, Mohd Yunus Abdul Shukor, Yook Heng Lee, Musa Ahmad |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Multidisciplinary Chromatography 010401 analytical chemistry chemistry.chemical_element 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Mercury (element) Amidase Hydrolysis chemistry.chemical_compound Ammonia Membrane chemistry Reagent Acrylamide 0210 nano-technology |
| Zdroj: | Sains Malaysiana. 46:1557-1563 |
| ISSN: | 0126-6039 |
| Popis: | In this work, a new optical screening method for acrylamide was developed. Bacterial Bacillus sp. strain ZK 34 was used to hydrolyse acrylamide to the corresponding acid and ammonia. Nessler’s reagent was used to detect the produced ammonia and the yellow complex formed was treated as signal. Bacterial pellet was immobilised in the alginate membrane. The optimum composition of alginate used is 2%. The mass ratio of alginate:bacterial of 1:0.5 gave the optimum respond. Optimum concentration for NaOH and Nessler’s reagent were 0.075 M and 2.5 mM, respectively. The yellow complex of mercury (II) amido-iodine formed was directly proportional to the concentrations of acrylamide up to 50.00 ppm with the limit of detection of 1.30 ppm. This sensor shows a good reproducibility which the relatives standard deviation (RSD) values from 3.17-6.15%. Therefore, the detection of acrylamide based on the amidase hydrolysis is suitable for screening this carcinogen compound. |
| Databáze: | OpenAIRE |
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