| Popis: |
Population genomics studies require the purification of high-quality DNA from field collected samples for subsequent analysis. Often it is impossible or impractical to snap freeze collected samples. Alternate storage methods have been used, such as preserving specimens in ethanol or DMSO. However, both are detrimental to the quality of the DNA recovered. We have found that dehydrating field-collected insect samples using silica gel is a simple, cheap and effective alternative. We can recover high quality DNA suitable for short-read sequencing approaches from samples that have been stored with silica gel for up to 8 weeks stored at ambient temperatures- suitable for the most remote field studies. Here we present a method for the construction of low-cost sample collection and storage tubes from commonly available laboratory consumables. |
