Eine spezifische Mittelstück-Färbung von Spermien und ihre mögliche Anwendung

Autor:	R. Weilenmann
Rok vydání:	2003
Předmět:	Andrology General Veterinary urogenital system Fresh semen biology.protein Horse Biology Positive correlation Staining technique Sperm motility Enzyme assay Staining
Zdroj:	Schweizer Archiv für Tierheilkunde. 145:233-237
ISSN:	1664-2848 0036-7281
DOI:	10.1024/0036-7281.145.5.233
Popis:	A special staining technique for the middle piece of sperms is described in different species (cow, horse, sheep, goat, pig). Staining was performed with frozen or fresh semen. The mitochondria in the middle piece could not always be stained with the same intensity. Using 600-fold magnification, subunits of the middle-piece could be observed, but only on fresh collected and immediately stained sperms. When kept in the refrigerator for more than 3 days, the observed subunits disappeared. A positive correlation between staining intensity and enzyme activity could be indicative for sperm motility and also fertilizing capacity.
Databáze:	OpenAIRE
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