Chromosomal studies after differential staining and fluorescence in situ hybridization using telomeric probe in three Leptodactylus species (Leptodactylidae, Anura)

Autor:	Renata Cecília Amaro-Ghilardi, Yatiyo Yonenaga-Yassuda, Miguel Trefaut Rodrigues
Rok vydání:	2004
Předmět:	Genetics biology medicine.diagnostic_test Differential staining Leptodactylidae Karyotype biology.organism_classification Leptodactylus Molecular biology Staining medicine Fish General Agricultural and Biological Sciences Fluorescence in situ hybridization
Zdroj:	Caryologia. 57:53-65
ISSN:	2165-5391 0008-7114
DOI:	10.1080/00087114.2004.10589372
Popis:	Leptodactylus ocellatus, L. knudseni and L. pentadactylus were cyto-genetically analysed following conventional and Ag-NOR staining, C- and R-banding and FISH using the sequence (TTAGGG)n as probe. These species show similar karyotypes with 2n = 22 and Ag-NORs located in the short arm of pair 8. Species-specific C-banding patterns were detected involving centromeric, telomeric and interstitial bands. The comparative study of chromosomes after R-banding allowed the establishment of homeologies among the six largest pairs and the NOR-bearing pair in all species. The sequence (TTAGGG)n was detected in the telomeric regions of the chromosomes and nowhere else.
Databáze:	OpenAIRE
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