Effects of omega-3 supplementation on markers of autophagy, apoptosis and mitochondrial area density

Autor: Megan Lo, Merryl Black, Chris McGlory, Joe Quadrilatero, Michaela Devries
Rok vydání: 2023
Předmět:
Zdroj: Physiology. 38
ISSN: 1548-9221
1548-9213
DOI: 10.1152/physiol.2023.38.s1.5735257
Popis: In humans, disuse is known to result in muscle atrophy and metabolic dysfunction, including reduced mitochondrial content. Previous data from this cohort found that omega-3 polyunsaturated fatty acid (n-3) supplementation attenuated declines in muscle mass, myofibrillar protein synthesis and ADP-stimulated mitochondrial respiration. Disuse has also been shown to increase apoptosis and events linked to autophagy. We investigated the effects of n-3 fatty acid supplementation on content of proteins involved in autophagy, intrinsic apoptosis, mitochondrial fission and fusion and mitochondrial area density during 14 d of immobilization and 14 d of recovery. Twenty females (18-30y) received 20mL doses of n-3 fatty acid (2.97g EPA and 2.03g DHA) or an isoenergetic sunflower oil (oleic acid content: 75%) daily during the 28-d protocol. Biopsies were taken from the vastus lateralis pre-immobilization, post-immobilization, and post-recovery. Protein and mitochondrial content were determined using Western Blot and transmission electron microscopy at 5800X magnification, respectively. Mitochondrial area density (%AD) decreased in the subsarcolemmal (SS, p=0.006) and central intramyofibrillar (IMF) (p=0.001) regions after recovery. The change in the central IMF was driven by a decrease in the n-3 group (p=0.0133). There was no change in the peripheral IMF region. P62 increased during immobilization (p This study was supported with a Canadian Institutes of Health Research Award to S.M. Phillips and a Natural Sciences and Engineering Research Council of Canada Award to M.C Devries This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Databáze: OpenAIRE
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