Pollen-expressed S-RNases are not involved in self-incompatibility in Lycopersicon peruvianum
| Autor: | Adrienne E. Clarke, Peter N. Dodds, Ed Newbigin, C. Ferguson |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Sexual Plant Reproduction. 12:76-87 |
| ISSN: | 1432-2145 0934-0882 |
| DOI: | 10.1007/s004970050175 |
| Popis: | Self-incompatibility in solanaceous plants is gametophytically controlled by a multiallelic S-locus. The only known S-locus product is a series of extracellular ribonucleases (the S-RNases) which are expressed in the mature style and determine its self-incompatibility phenotype. Potentially, S-RNases also determine the self-incompatibility phenotype of pollen as some solanaceous plants express this protein for a brief period during anther development. To test this, we first showed that the S3-RNase of Lycopersicon peruvianum is expressed during anther development. We then transformed L. peruvianum plants with sense and antisense versions of the S3-RNase coding region under the control of a pollen-specific promoter. Pollen from the transgenic plants accumulated S3-RNase transcripts and the S3-RNase protein was detected immunologically in the sense transgenic plants. However, neither the sense nor the antisense S3-RNase constructs altered the self-incompatibility phenotype of pollen from the transgenic plants, demonstrating the S3-RNase is not the pollen product of the S3-allele. |
| Databáze: | OpenAIRE |
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