Retinoic acid-induced granulocytic differentiation of HL-60 myeloid leukemia cells is mediated directly through the retinoic acid receptor (RAR-alpha)

Autor:	Steven J. Collins, Kent A. Robertson, LeMoyne Mueller
Rok vydání:	1990
Předmět:	Myeloid Cellular differentiation Retinoic acid Myeloid leukemia Cell Biology Biology medicine.disease Leukemia chemistry.chemical_compound Retinoic acid receptor medicine.anatomical_structure chemistry Cancer research medicine Receptor Molecular Biology Transcription factor
Zdroj:	Molecular and Cellular Biology. 10:2154-2163
ISSN:	1098-5549 0270-7306
DOI:	10.1128/mcb.10.5.2154
Popis:	Retinoic acid (RA) induces terminal granulocytic differentiation of the HL-60 promyelocytic leukemia cell line as well as certain other human myeloid leukemias. Specific RA receptors that are members of the steroid-thyroid hormone superfamily of nuclear transcription factors have recently been identified. We developed an HL-60 subclone that was relatively resistant to RA-induced differentiation. Specific nuclear RA receptors in this RA-resistant subclone had a decreased affinity for RA and exhibited a lower molecular weight compared with nuclear RA receptors from the RA-sensitive parental HL-60 cells. Retroviral vector-mediated transduction of a single copy of the RA receptor (RAR-alpha) into this RA-resistant HL-60 subclone restored the sensitivity of these cells to RA. These observations indicate that RAR-alpha plays a critical and central role in mediating RA-induced terminal differentiation of HL-60 leukemia cells.
Databáze:	OpenAIRE
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