Abstract 141: Histone Methyltransferase Setdb2 is important for miRNA mediated cardiac reprogramming
| Autor: | Imke Kirste, Tilanthi M Jayawardena, J. A Payne, Victor J Dzau, Maria Mirotsou |
|---|---|
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Circulation Research. 113 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/res.113.suppl_1.a141 |
| Popis: | Rationale: Regeneration of damaged cardiac tissue after injury presents a daunting challenge in cardiovascular medicine. Recent developments in reprogramming of somatic cells directly to cells of other lineages have raised the possibility of using this approach for cardiac regenerative therapy. Our group recently demonstrated successful miRNA mediated cardiac reprogramming in vitro and in vivo using a combination of miRNAs 1, 133, 206 and 499. Although, the molecular mechanisms underlying miRNA mediated fibroblast reprogramming to cardiomyocytes are yet unknown, accumulating evidence suggest that reprogramming acts through distinct phases and that histone modifications play an important role in these processes. Objective: Identify key genes involved in initiating miRNA mediated reprogramming via histone modifications. Methods and Results: For this, we analyzed the expression levels of 81 different genes involved in chromatin modification 4 days after miRNA transfection using PCR arrays. This analysis revealed that 6 of the 81 tested genes showed differential gene expression (≤-1.5-fold and p Conclusion: In conclusion our results indicate that Setdb2 down-regulation plays an important role in the direct reprogramming of fibroblasts to cardiomyocyte-like cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|