Large scale extraction of high quality moss DNA

Autor:	Franz Mittmann, S. Dienstbach, G. Wagner
Rok vydání:	2007
Předmět:	Ceratodon purpureus biology Botany Gene targeting Plant Science Homologous recombination Physcomitrella patens biology.organism_classification DNA extraction Gene Gene knockout Southern blot
Zdroj:	Russian Journal of Plant Physiology. 54:564-568
ISSN:	1608-3407 1021-4437
Popis:	For several years Physcomitrella patens Hedw. was the only land plant allowing targeted gene knockout via homologous recombination, which provides an efficient and elegant tool for the analysis of gene functions. The moss Ceratodon purpureus Hedw. was recently shown to have similar capabilities. However, extraction of high quality total DNA from both moss species — necessary for unambiguous proof of successful gene targeting events — is still a severe problem. Here, we report an improved DNA isolation protocol for moss filaments, which is fast, reliable, cheap, quick, and easy. It results in satisfying yields of DNA suitable for PCR and Southern blotting. The modified extraction procedure was additionally tested successfully for the alga Mougeotia scalaris Hass. as well as the higher plant Arabidopsis thaliana L.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d1c59cfac3c3a5852b6e2168f06ff91d https://doi.org/10.1134/s1021443707040206 Zobrazit plný text záznamu Plný text ve formátu PDF