Study on Effects of Ag-SiO2 Core Shell Nanoparticles on Biocompatibility Appraisal of Myoblasts
Autor: | Touseef Amna, Myung-Seob Khil, Rizwan Khan, M. Shamshi Hassan, Abdullah A. A. Alghamdi |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Materials science Biocompatibility Cell Biology 01 natural sciences Agricultural and Biological Sciences (miscellaneous) In vitro law.invention 03 medical and health sciences Silver nitrate chemistry.chemical_compound 030104 developmental biology chemistry Transmission electron microscopy Confocal microscopy law Genetics Biophysics Myocyte Cytotoxicity C2C12 010606 plant biology & botany |
Zdroj: | Cytology and Genetics. 55:199-204 |
ISSN: | 1934-9440 0095-4527 |
Popis: | The main goal of present study was to check interaction of Ag-SiO2 core shell nanoparticles (CSNs) with C2C12 cells. We herein account the synthesis and classification of novel CSNs. We aimed the preparation of CSNs in order to check their biocompatible/cytotoxic impact on C2C12 muscle cells. We aimed to check the CSNs related hazards to human health. The utilized CSNs were synthesized by the straightforward sol–gel method utilizing silver nitrate and tetraethoxysilane as fundamental components. The physicochemical characterization of the CSNs was conceded by means of X-ray diffraction, UV-Vis and transmission electron microscopy. To examine in vitro biocompatibility/cytotoxicity, C2C12 cells were cultured under in vitro environment and afterward subjected to different concentrations of CSNs. The survival of C2C12 cells was evaluated via cell counting Kit-8 assay at exact point gap. These results were authenticated by confocal microscopy. The morphology of C2C12 cells was observed with the help of a phase contrast microscope. In vitro investigation of biological effects of CSNs has revealed that feasibility of cells in culture follows dose (0–20 μg/mL) mode. Overall, results of this investigation demonstrate that synthetic CSNs influence C2C12 cell practicability with time point as well as absorption dependent mode. |
Databáze: | OpenAIRE |
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