Development of SNP markers for Xenocypris argentea based on transcriptomics
| Autor: | Jun Liu, Xusheng Guo, Peng Xinliang, Liangjie Zhao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Genetics Molecular breeding education.field_of_study Population Biodiversity Single-nucleotide polymorphism Biology 010603 evolutionary biology 01 natural sciences Transcriptome 03 medical and health sciences 030104 developmental biology Genetic linkage SNP education Ecology Evolution Behavior and Systematics |
| Zdroj: | Conservation Genetics Resources. 10:679-684 |
| ISSN: | 1877-7260 1877-7252 |
| Popis: | Xenocypris argentea is a cyprinid fish species and widely distributed in rivers and lakes within China. The germplasm resources of X. argentea have recently been seriously degraded by human activities. Thus, the development of sufficient molecular markers to inform conservation efforts of X. argentea is urgently needed. In this study, we used Illumina HiSeq high-throughput sequencing of X. argentea transcriptomic data to identify single nucleotide polymorphism (SNP) markers for X. argentea. From 43,346 unigenes with functional annotations, 462,621 putative SNPs were identified. Among these, we validated 32 polymorphic loci of 40 function-related SNPs from a Poyang Lake population of X. argentea. Six of these SNP loci deviated significantly from Hardy–Weinberg equilibrium (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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