Determination of Gatifloxacin in Semen by HPLC with Diode-Array and Fluorescence Detection
| Autor: | N. Waksman de Torres, L. Y. Colunga González, G. Gallegos de Lerma, M. L. Salazar Cavazos |
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| Rok vydání: | 2006 |
| Předmět: |
Chromatography
Chemistry Organic Chemistry Clinical Biochemistry Semen Biochemistry Fluorescence High-performance liquid chromatography Gatifloxacin Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound medicine Citric acid Quantitative analysis (chemistry) Antibacterial agent medicine.drug |
| Zdroj: | Chromatographia. 63:605-608 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1365/s10337-006-0807-9 |
| Popis: | The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 ± 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 μg.mL−1 respectively, with RSD of 3.4% for DAD and 2.8% for fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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