| Autor: |
Peng Zhang, Guangwei Wu, Stephanie C. Heard, Changshan Niu, Stephen A. Bell, Fengli Li, Ying Ye, Yonghui Zhang, Jaclyn M. Winter |
| Rok vydání: |
2022 |
| Popis: |
We report the identification of the tnd biosynthetic cluster from the marine-derived fungal strain Aspergillus flavipes CNL-338 and in vivo characterization of a cryptic type I diterpene synthase. Heterologous expression of the bifunctional terpene synthase TndC in Saccharomyces cerevisiae led to the discovery of a new diterpene backbone, talarodiene, harboring a benzo[a]cyclopenta[d]cyclooctane tricyclic fused ring system. The cyclization mechanism that converts geranylgeranyl diphosphate to the tricyclic hydrocarbon skeleton was investigated using 13C-labeling studies and stable isotope tracer experiments showed the biotransformation of talarodiene into talaronoid C. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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