A comparative study of in vitro contractility between gut tissues of Hirschsprung's disease and other gut malformations
| Autor: | Phani Bhushan Singh, Sanjeev K. Singh, R. P. Pandey, Maloy B Mandal, Anil Kumar Tiwari, Ajay N. Gangopadhyay, Shashikant C.U. Patne |
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| Rok vydání: | 2017 |
| Předmět: |
Agonist
congenital hereditary and neonatal diseases and abnormalities medicine.medical_specialty Physiology business.industry medicine.drug_class Histamine H1 receptor medicine.disease digestive system Gastroenterology digestive system diseases Contractility chemistry.chemical_compound Atropine Endocrinology chemistry Internal medicine medicine General Pharmacology Toxicology and Pharmaceutics business Hirschsprung's disease H1 antagonist Acetylcholine Histamine medicine.drug |
| Zdroj: | National Journal of Physiology, Pharmacy and Pharmacology. :1 |
| ISSN: | 2320-4672 |
| DOI: | 10.5455/njppp.2017.7.0623205072017 |
| Popis: | Background: Hirschsprungs disease and other gut malformations commonly present with obstructive features of gut in pediatric age group. Problem of obstruction persists even after resection and anastomosis operation. Aims and Objectives: This in vitro comparative study was performed to assess the gut contractility to chemical mediators such as Acetylcholine and Histamine between Hirschsprungs disease and other gut malformations. Materials and Methods: The longitudinal muscle strips of Hirschsprungs cases and other gut malformations (non-Hirschsprungs cases) were placed in Dales organ bath containing Krebs-Ringer solution, continuously bobbled with 100% O2 at 28°C. Gut contractions were recorded using Power Lab 4/ST system and was analyzed using software CHART-5 for windows. Control contractions were recorded against initial tension of 0.5 g. Subsequently, agonist (acetylcholine, histamine)-induced contractions were recorded before and after appropriate antagonists (atropine, pheniramine). Before values of agonist-induced contractions of Hirschsprung and non-Hirschsprungs cases were compared. Values of agonistinduced contractions obtained after pretreatment with antagonists were also compared between Hirschsprung and non- Hirschsprungs cases. Results: Acetylcholine enhanced contractions in non-Hirschsprungs cases and it caused small increase in amplitude of contractions in Hirschsprungs cases. Atropine pretreatment blocked acetylcholine-induced contractions significantly in non-Hirschsprungs cases, whereas it failed to block in the Hirschsprungs cases. Histamine augmented contractions in both Hirschsprung and non-Hirschsprungs cases. H1 antagonist, pheniramine failed to block the contractility in both the cases. Conclusion: Findings of this study suggested that acetylcholine increased gut contractility significantly in non-Hirschsprungs cases involving muscarinic-cholinergic pathways, whereas histamine increased gut contractility in both Hirschsprungs disease and non-Hirschsprungs cases and it is not mediated by H1 receptors. |
| Databáze: | OpenAIRE |
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