Effect of protriptyline on [Ca2+]iand viability in MG63 human osteosarcoma cells
| Autor: | Fu-An Chen, Wei-Zhe Liang, Pochuen Shieh, Hong-Tai Chang, Daih-Huang Kuo, Jin-Shiung Cheng, I-Shu Chen, Chiang-Ting Chou, Chia-Cheng Yu, Ko-Long Lin, Chun-Chi Kuo, Ching-Kai Su, Chung-Ren Jan, Ti Lu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Thapsigargin Econazole business.industry Health Toxicology and Mutagenesis Endoplasmic reticulum Cancer Protriptyline Toxicology medicine.disease 03 medical and health sciences Cytosol chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Endocrinology chemistry Nifedipine 030220 oncology & carcinogenesis Internal medicine Medicine Osteosarcoma business medicine.drug |
| Zdroj: | Toxicology Mechanisms and Methods. 26:580-587 |
| ISSN: | 1537-6524 1537-6516 |
| DOI: | 10.1080/15376516.2016.1216208 |
| Popis: | Tricyclic antidepressants (TCA) have been clinically prescribed in the auxiliary treatment of cancer patients. Although protriptyline, a type of TCA, was used primarily in the clinical treatment of mood disorders in cancer patients, the effect of protriptyline on physiology in human osteosarcoma is unknown. This study examined the effect of protriptyline on cytosolic free Ca2+ concentrations ([Ca2+]i) and viability in MG63 human osteosarcoma cells. Protriptyline between 50 and 250 μM evoked [Ca2+]i rises concentration-dependently. Protriptyline induced influx of Mn2+, indirectly implicating Ca2+ influx. Protriptyline-evoked Ca2+ entry was inhibited by nifedipine by 20% but was not altered by econazole, SKF96365, GF109203X, and phorbol-12-myristate-13-acetate (PMA). In Ca2+-free medium, treatment with protriptyline inhibited the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin-evoked [Ca2+]i rises. Conversely, treatment with thapsigargin inhibited 45% of protriptyline-evoked [Ca2+]i rises. In... |
| Databáze: | OpenAIRE |
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